Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

The western blot is a method for detecting the presence and size of specific proteins. Proteins are first separated according to size or other physical properties by gel electrophoresis, then ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

GE Healthcare Life Sciences recently launched the Amersham WB system for quantitative SDS-PAGE and Western blotting of proteins using fluorescence detection. The system was standardized for the entire ...

Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

Meet the trusted FluorChem TM Western blot imaging systems. Simply load your sample, choose a protocol, and hit expose. Load less protein, use smaller amounts of your precious antibodies, and never ...

The western blot may appear to be just another staple lab technique. However, there are some stories surrounding its invention that are definitely worth knowing. This might be news to some: scientists ...

A classic laboratory technique, western blotting, has been used by researchers for decades, and it is nearly obsolete. "Biologists currently are stuck in a time warp—they basically use the same tools ...