Electrophoretic mobility shift assays (EMSAs) are commonly used to analyze nucleic acid–protein interactions. When nucleic acid is bound by protein, its mobility during gel electrophoresis is reduced.

Here we describe the protocols for negative or reverse detection of proteins, nucleic acids and lipopolysaccharides separated in polyacrylamide electrophoresis gels. These protocols are based on the ...

What are the basic principles of electrophoresis? In electrophoresis, the substance that has to be separated is made to travel across a gel with a specific pore size using an electrical field. Also, ...