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26:58
YouTube
Andrew Douch
Making synthetic human insulin
In this video we explore the traditional way that synthetic, recombinant human insulin is produced, by synthesising the A and B chains separately in separate strains of E. coli, and then combined in vitro to make insulin. We look at how ampicillin and tetracycline resistance genes, and blue-white screening with X-gal are used to identify ...
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